Avaliação da aderência in vitro do streptococcus mutans à superfície de braquetes ortodônticos linguais
Brunela Machado Lima.
Dissertação
Português
D4
2014.
41 f. : il.
Dissertação (Mestrado em Ortodontia) - Centro de Pesquisas Odontológicas São Leopoldo Mandic.
Os braquetes linguais voltaram para o cenário ortodôntico com novas técnicas e desings favorecendo sua aplicação clínica. Muito se fala da estética nesta técnica, porém a literatura ainda é escassa quanto a aderência bacteriana na superfície desses braquetes. O objetivo do presente trabalho foi...
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Os braquetes linguais voltaram para o cenário ortodôntico com novas técnicas e desings favorecendo sua aplicação clínica. Muito se fala da estética nesta técnica, porém a literatura ainda é escassa quanto a aderência bacteriana na superfície desses braquetes. O objetivo do presente trabalho foi analisar a aderência do Streptococcus mutans (ATCC 25175) à superfície de diferentes braquetes linguais na ausência e presença de sacarose. A amostra incluiu 12 braquetes metálicos de 05 marcas comerciais diferentes, totalizando 60 braquetes. Três grupos contendo soluções distintas foram formados: S1 (2 mL de caldo BHI e 50 ?L do inóculo), S2 (2 mL de caldo BHI com sacarose e 50 ?L do inóculo) e S3 (2 mL de caldo BHI com sacarose - controle negativo). Cada grupo recebeu 20 braquetes, que foram inseridos individualmente em tubos de ensaio com a solução referente ao seu grupo e incubados a 37ºC/24h em estufa de CO2. Na sequência, foram transferidos para tubos de Eppendorf contendo 1ml de solução salina estéril, homogeneizados por 01 minuto em agitador de soluções e realizadas diluições seriadas até 10-5. Alíquotas de 25µl de cada diluição foram inoculadas em triplicata em placas de Petri contendo meio de cultura MAS (Ágar Mitis Salivarius) pela técnica da gota. As placas foram incubadas a 37ºC/72h em estufa de CO2 para posterior contagem das UFC/mL. O teste de Kruskal-Wallis não demonstrou diferença significativa na contagem de Streptococcus mutans quando a amostra foi avaliada em S1 (p = 0,059). O mesmo resultado foi verificado na solução S2 (p = 0,733). Testes de Mann-Whitney revelaram que para os braquetes Prieto (Tecnident®; p = 0,773) e 2D (Forestadent®; p = 0,248) não foi observada diferença significativa na contagem bacteriana nas soluções S1 e S2. Já para os braquetes STB (Ormco®; p = 0,043), In-Ovation L (GAC®; p = 0,021) e Tecnident Standart (Tecnident®; p = 0,030) foi constatada menor contagem na solução S2. Conclui-se que a aderência do Streptococcus mutans foi similar quando os diferentes braquetes linguais foram comparados entre si.
Palavras-chave: Braquetes ortodônticos. Adesão bacteriana. Técnica lingual Streptococcus mutans.
Lingual brackets are back to the orthodontic scene with innovated techniques and designs which enhance its clinical application. Much is discussed about the aesthetic in this technique, however the literature still lack evidence regarding bacterial adhesion on the surface of these brackets. The objective of this study was to analyze the adherence of Streptococcus mutans (ATCC 25175) on the surface of different lingual brackets in the absence and presence of sucrose. The sample included 12 metal brackets of 05 different commercial brands, totaling 60 brackets. Three groups were formed containing distinct solutions: S1 (2 ml of BHI broth and 50 of ?l inoculum ), S2 ( 2 mL of BHI broth with sucrose and 50?l of inoculum) and S3 ( 2 mL of BHI broth with sucrose - negative control). Each group received 20 brackets, which were placed individually in test tubes with the solution related to their group and incubated at 37 ° C/24h in CO2 incubator. Following, they were transferred to Eppendorf tubes containing 1 ml of sterile saline, then homogenized for 01 minutes on shaker and made solutions and dilutions to 10-5. Aliquots of 25?l of each dilution were inoculated in triplicate petri dishes containing culture medium MAS (Mitis salivarius agar) using the drop technique . The plates were incubated at 37 ° C/72h in CO2 incubator for later counting the CFU / ml. The Kruskal -Wallis test showed no significant difference in the count of Streptococcus mutans when the sample was evaluated in S1 (p = 0.059). The same result was observed in S2 ( p = 0.733 ) solution.The Mann-Whitney tests revealed that for the Prieto brackets ( Tecnident ® p = 0.773 ) and 2D ( Forestadent ® p = 0.248 ) were observed no significant difference in bacterial count in the solutions S1 and S2 . As for the STB brackets ( Ormco ® , p = 0.043 ) , In-Ovation L ( GAC ® , p = 0.021 ) and Tecnident Standart ( Tecnident ® , p = 0.030 ) was found lower counts in S2 solution. We conclude that the adhesion of Streptococcus mutans was similar when different lingual brackets were compared among each other.
Keywords: Orthodontic brackets. Bacterial adhesion. Streptococcus mutans lingual technique Ver menos
Palavras-chave: Braquetes ortodônticos. Adesão bacteriana. Técnica lingual Streptococcus mutans.
Lingual brackets are back to the orthodontic scene with innovated techniques and designs which enhance its clinical application. Much is discussed about the aesthetic in this technique, however the literature still lack evidence regarding bacterial adhesion on the surface of these brackets. The objective of this study was to analyze the adherence of Streptococcus mutans (ATCC 25175) on the surface of different lingual brackets in the absence and presence of sucrose. The sample included 12 metal brackets of 05 different commercial brands, totaling 60 brackets. Three groups were formed containing distinct solutions: S1 (2 ml of BHI broth and 50 of ?l inoculum ), S2 ( 2 mL of BHI broth with sucrose and 50?l of inoculum) and S3 ( 2 mL of BHI broth with sucrose - negative control). Each group received 20 brackets, which were placed individually in test tubes with the solution related to their group and incubated at 37 ° C/24h in CO2 incubator. Following, they were transferred to Eppendorf tubes containing 1 ml of sterile saline, then homogenized for 01 minutes on shaker and made solutions and dilutions to 10-5. Aliquots of 25?l of each dilution were inoculated in triplicate petri dishes containing culture medium MAS (Mitis salivarius agar) using the drop technique . The plates were incubated at 37 ° C/72h in CO2 incubator for later counting the CFU / ml. The Kruskal -Wallis test showed no significant difference in the count of Streptococcus mutans when the sample was evaluated in S1 (p = 0.059). The same result was observed in S2 ( p = 0.733 ) solution.The Mann-Whitney tests revealed that for the Prieto brackets ( Tecnident ® p = 0.773 ) and 2D ( Forestadent ® p = 0.248 ) were observed no significant difference in bacterial count in the solutions S1 and S2 . As for the STB brackets ( Ormco ® , p = 0.043 ) , In-Ovation L ( GAC ® , p = 0.021 ) and Tecnident Standart ( Tecnident ® , p = 0.030 ) was found lower counts in S2 solution. We conclude that the adhesion of Streptococcus mutans was similar when different lingual brackets were compared among each other.
Keywords: Orthodontic brackets. Bacterial adhesion. Streptococcus mutans lingual technique Ver menos
Avaliação da aderência in vitro do streptococcus mutans à superfície de braquetes ortodônticos linguais
Brunela Machado Lima.
Avaliação da aderência in vitro do streptococcus mutans à superfície de braquetes ortodônticos linguais
Brunela Machado Lima.
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